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Lentiviral are vectors that are derived from the virus known as human immunodeficiency virus and is used to transfer gene in the cells, such as mammalian cell and others. Lentiviruses are used for tra...


Lentiviral are vectors that are derived from the virus known as human immunodeficiency virus and is used to transfer gene in the cells, such as mammalian cell and others. Lentiviruses are used for transfection in non-dividing and actively dividing cell. This acts as an advantage to another type of retrovirus. The lentiviruses fall in the category of a retrovirus. The lentiviral expression system is developed, which can influence the gene of interest. The commercially available lentiviral expression systems are available in combination with tag sequence, reporter gene, promoters and multicistronic vectors are also available for desired protein expression. These vectors are used for research and development purpose recombinant protein expression, protein characterization Wholesale Xavier Woods Jersey , in-vitro transcription, and other functional arrays.


The HIV vector is used as an attractive transfection vector and in use in research laboratories in biotechnological and pharmaceutical companies. The lentiviral expression system is highly effective gene therapy and is designed to optimize high expression and high titers for use in the mammalian cell, primary cells, nondividing cells, stem cells, and neurons. The lentiviral wraps up to 5kb of foreign DNA. The transduction with lentivirus vector is highly efficient, so the concern on the multiplicity of infection (MOI) should be taken into account. For cells to be infected by only one type of lentiviral particle Wholesale Jourdan Lewis Jersey , the MOI should be less than one. The screening rate for transfection is mostly 100% after the transfected cells are screened for antibiotic resistance or fluorescent screening technique